Bed Bug Protocol

Cryonite® Service Guidelines for Bed Bugs in Hotels/Motels

Bed bugs are accomplished hitch-hikers and can be transferred anywhere people may travel including buses, trains, and airplanes, hospitals, schools, homes, offices, theaters, and public buildings. Cryonite is ideally suited for bed bugs because it can be used in most any environment on most every type of surface. People and pets can remain in areas being treated with Cryonite, though it is recommended treatment areas be vacated as often residual treatments will be made following Cryonite application.

Bed bugs are difficult to control but are highly susceptible to being killed by direct contact with Cryonite freezing technology. Successful control of these pests is multifaceted and includes the following:

1. Inspection.

  • Bed bugs prefer to spend most of their time hiding within cracks and voids. They emerge at night to feed, and most bed bugs will be found in and around beds (70%), upholstered furniture (23%) and then a scattering of bugs in other areas (7%).
  • Carefully take apart the bed, remove drawers from furniture and check all sides, edges, cracks and folds for live bugs, eggs, and fecal spots.

2. Physically remove as many live bedbugs as possible with a vacuum.

  • Vacuuming provides immediate population reduction.
  • Vacuuming does not dislodge or remove eggs.

3. Prepare the Cryonite equipment for application.

  • Is the Cryonite Lance and Hose hooked up to a CO2 tank with a dip tube?
  • All connections are tight and not leaking?
  • System is producing dry ice snow as designed?

4. Use Cryonite to treat surfaces and cracks of all identified and suspected sites with bed bug activity on beds, furniture, and other items.


  • Remove the Jet Nozzle from the Lance for application to surfaces.
  • Apply a light layer of dry ice snow along all mattress seams, under flaps and folds and to other surfaces on and around the bed and bed frame.
    • Hold tip of Lance about 10-12 inches above the target surface.
    • Depress handle on the Lance about half-way. Avoid depressing handle completely!
    • Make several quick passes over the target surface(s) to build a layer of CO2 snow.

For best results, the layer of snow should evaporate completely within 20 to 30 seconds.

  • Apply dry ice snow to backs, under drawers and the bottom of night stands and other furniture.
  • Examine toys, books, DVDs, and other items for evidence of bed bugs and treat any activity found. Cryonite may be used on almost every type of surface. (Refer to Cryonite Manual for more details.
  • Cracks may be treated using the Jet Nozzle (for cracks & crevices). DO NOT use the Jet Nozzle to treat surfaces!  You will only blow bed bugs and debris onto other areas. 

When using the Jet Nozzle, only depress the handle on Cryonite Lance about half way (50%). Applying at full pressure wastes gas and blows insects and debris around, allowing some insects to survive.

5. When treating into voids that may contain bed bugs, using the Jet Nozzle tip.


  • Application of Cryonite can help kill bugs hidden in voids.
  • Apply about 5 seconds per application site into voids.
  • The CO2 produced when the dry ice snow evaporates will trigger bed bugs to become active and many will crawl out of cracks and voids where they can be removed by vacuum or treated directly with Cryonite.
  • If insects are present within the void, follow the Cryonite application with a void treatment using an application of residual insecticide dust.

6. Where time allows, vacuum.

  • Vaccuum bed bugs flushed into the open about 15-20 minutes after Cryonite application.
  • Cryonite will cause bed bugs to leave cracks and harborages so a follow-up to vacuum assists with immediate population reduction.

7. Apply residual insecticide treatments.

  • Use appropriately labeled dust, aerosol, or water-based formulations to cracks, voids and surfaces where bed bug activity was found or is suspected.
  • Residual treatments kill bed bugs missed by vacuuming or Cryonite applications, particularly those hidden within voids or other less accessible sites.
  • Follow products’ label directions for treating bed bugs. For example, some product labels have
  • Use Restrictions that may prevent of limit applications to certain sites, such as mattresses and areas of upholstered furniture that may be contacted frequently by people.


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